Riboflavin along with antibiotics balances reactive oxygen species and inflammatory cytokines and controls Staphylococcus aureus infection by boosting murine macrophage function and regulates inflammation

Table 1 Riboflavin concentration inside the macrophages (μg/ml)

Samples	Concentrations of riboflavin uptake (μg/ml)
Samples	60 min	90 min
CM + RIBO	37.31724 ± .551	38.21379 ± .482
RIBO + SAM	46.48966 ± 1.724^a	46.35172 ± 1.655^a
RIBO + AZM + SAM	46.48966 ± 1.724^a	54.97241 ± .1379^b
RIBO + CIP + SAM	52.76552 ± 0.689^b	57.31724 ± .413^b

Riboflavin concentrations inside the macrophages were estimated from the cell lysate by spectrophotometric analysis and were expressed in μg/ml. Results were shown as mean ± SD of three independent experiments
CM + RIBO Riboflavin pre-treated Control macrophage, RIBO + SAM, Riboflavin pretreated + S. aureus infected macrophages, RIBO + AZM + SAM Riboflavin and Azithromycin pretreated macrophages infected with S. aureus, RIBO + CIP + SAM Riboflavin and Ciprofloxacin pretreated macrophages infected with S. aureus
^asignificant difference with respect to CM + RIBO
^bsignificant difference with respect to Riboflavin (RIBO+SAM)

ISSN: 1476-9255