Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest

Table 1 Comparison of tissue total alkaline phosphatase (AP) activity by intervention group

Tissue	Intervention Group Total AP Activity (U/L) Mean (SD)					P value (ANOVA)	Significant Post-HOC Group Comparisons
Tissue	AC (0)	CPB/DHCA (1)	CPB/DHCA + AP Low (2)	CPB/DHCA + AP Medium (3)	CPB/DHCA + AP High (4)	P value (ANOVA)	Significant Post-HOC Group Comparisons
Kidney	2211 (491)	1628 (472)	1536 (441)	1404 (165)	2028 (318)	0.01	0 vs. 1; 0 vs. 2; 0 vs. 3; 3 vs. 4
Liver	598 (115)	642 (161)	725 (100)	609 (161)	875 (146)	0.02	0 vs. 4; 1 vs. 4; 3 vs. 4
Jejunum	2160 (1162)	1460 (663)	1760 (477)	2292 (586)	2060 (877)	0.51
Ileum	1270 (432)	1016 (368)	982 (508)	1450 (1135)	1312 (332)	0.71
Colon	658 (327)	664 (435)	500 (97)	540 (238)	358 (185)	0.40
Lung-Apex	163 (79)	263 (153)	305 (108)	276 (82)	206 (57)	0.17
Lung-Lower Lobe	117 (46)	137 (21)	216 (85)	280 (38)	201 (57)	0.0003	0 vs. 2; 0 vs. 3; 0 vs. 4; 1 vs. 2; 1 vs. 3; 3 vs. 4
Heart	40 (12)	47 (15)	51 (19)	50 (10)	48 (8)	0.57

Group 0 = Anesthesia controls (AC); Group 1 = Animals undergoing CBP/DHCA; Group 2 = Animals undergoing CPB/DHCA with low dose BiAP (3 U/kg bolus followed by 1 U/kg/hr. infusion); Group 3 = Animals undergoing CPB/DHCA with medium dose BiAP (15 U/kg bolus followed by 5 U/kg/hr. infusion); Group 4 = Animals undergoing CPB/DHCA with high dose BiAP (75 U/kg bolus followed by 25 U/kg/hr. infusion). Data are expressed as means (SD). One-way ANOVA was performed followed by post-hoc Student’s t-tests as appropriate. Bold: significant at p < 0.05

ISSN: 1476-9255