Tissue alkaline phosphatase activity and expression in an experimental infant swine model of cardiopulmonary bypass with deep hypothermic circulatory arrest

Table 2 Comparison of tissue non-specific alkaline phosphatase (TNAP) mRNA expression

Tissue	TNAP Relative mRNA Levels by Intervention Group Median (Intra-Quartile Range)					P value (Kruskal-Wallis)
Tissue	AC (0)	CPB/DHCA (1)	CPB/DHCA + AP Low (2)	CPB/DHCA + AP Medium (3)	CPB/DHCA + AP High (4)	P value (Kruskal-Wallis)
Kidney	1.03 (0.73–1.61)	1.42 (0.80–1.91)	0.65 (0.022–1.51)	1.00 (0.67–1.48)	0.80 (0.33–1.25)	0.51
Liver	1.01 (0.78–1.75)	1.28 (0.83–1.82)	0.94 (0.52–1.71)	1.67 (1.31–2.55)	1.48 (0.81–1.74)	0.42
Jejunum	1.00 (0.74–1.93)	1.35 (1.11–2.21)	1.56 (0.99–1.82)	1.13 (1.07–1.21)	1.07 (0.78–1.32)	0.41
Ileum	1.00 (0.25–2.11)	0.21 (0.15–3.84)	0.74 (0.58–0.81)	0.35 (0.19–0.58)	0.38 (0.16–0.53)	0.22
Colon	1.00 (0.70–1.33)	0.81 (0.69–1.55)	0.73 (0.47–1.20)	0.65 (0.39–0.84)	0.82 (0.80–0.99)	0.29
Lung-Apex	1.12 (0.41–2.47)	6.14 (4.02–11.06)	5.47 (1.65–19.57)	5.46 (1.87–9.61)	3.99 (1.91–5.40)	0.089
Lung-Lower Lobe	1.57 (1.04–2.13)	5.08 (2.44–13.11)	6.36 (4.95–11.76)	6.60 (1.19–9.21)	6.68 (3.62–7.94)	0.067
Heart	1.00 (0.45–1.64)	1.09 (0.64–1.36)	1.43 (0.85–1.53)	0.84 (0.71–1.72)	1.57 (0.55–2.32)	0.89

Group 0 = Anesthesia controls (AC); Group 1 = Animals undergoing CBP/DHCA; Group 2 = Animals undergoing CPB/DHCA with low dose BiAP (3 U/kg bolus followed by 1 U/kg/hr. infusion); Group 3 = Animals undergoing CPB/DHCA with medium dose BiAP (15 U/kg bolus followed by 5 U/kg/hr. infusion); Group 4 = Animals undergoing CPB/DHCA with high dose BiAP (75 U/kg bolus followed by 25 U/kg/hr. infusion). Data are expressed as medians with intra-quartile ranges. Kruskal-Wallis test was performed

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