Articles

Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication

This study was designed to evaluate the protective effects of thalidomide on paraquat (PQ)-induced lung injuries in a rat model and to explore the underlying mechanisms.

Authors: Dan Li, Xiao-Wei Zhang, Xing-Quan Jiang, Yong-Jie Yin, Zhe Fan, Cheng-Bo Sun, Xing-Hai Chen, Yan-Hui Li and Ke-Xiang Liu

A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers

Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was develop...

Authors: Tom Nilsen, Kathrin Sunde and Anders Larsson

Coupled cell networks are target cells of inflammation, which can spread between different body organs and develop into systemic chronic inflammation

Several organs in the body comprise cells coupled into networks. These cells have in common that they are excitable but do not express action potentials. Furthermore, they are equipped with Ca²⁺ signaling systems...

Authors: Elisabeth Hansson and Eva Skiöldebrand

Interleukin-18 expression increases in response to neurovascular damage following soman-induced status epilepticus in rats

Status epilepticus (SE) can cause neuronal cell death and impaired behavioral function. Acute exposure to potent acetylcholinesterase inhibitors such as soman (GD) can cause prolonged SE activity, micro-hemorr...

Authors: Erik A. Johnson, Michelle A. Guignet, Thuy L. Dao, Tracey A. Hamilton and Robert K. Kan

Functional regulation of Zfp36l1 and Zfp36l2 in response to lipopolysaccharide in mouse RAW264.7 macrophages

The tristetraprolin (TTP) family of mRNA-binding proteins contains three major members, Ttp, Zfp36l1, and Zfp36l2. Ttp down-regulates the stability of AU-rich element–containing mRNAs and functions as an anti-...

Authors: Kuan-Ting Wang, Hsin-Hui Wang, Yan-Yun Wu, Yu-Lun Su, Pei-Yu Chiang, Nien-Yi Lin, Shun-Chang Wang, Geen-Dong Chang and Ching-Jin Chang

Increased expression of the NLRP3 inflammasome components in patients with Behçet’s disease

Behçet’s disease (BD) is a systemic inflammatory disease with manifestations including recurrent oral and genital ulcerations, and vasculitis involving the skin, mucosa, joints, eyes, veins, arteries, nervous ...

Authors: En Hyung Kim, Mi-Jin Park, Sun Park and Eun-So Lee

Nur77 deficiency leads to systemic inflammation in elderly mice

Nur77, an orphan member of the nuclear receptor superfamily, has been implicated in the regulation of inflammation. However, the in vivo function of Nur77 remains largely unexplored. In the current study, we inv...

Authors: Xiu-Ming Li, Xing-Xing Lu, Qian Xu, Jing-Ru Wang, Shen Zhang, Peng-Da Guo, Jian-Ming Li and Hua Wu

Δ⁹-Tetrahydrocannabinol (THC) enhances lipopolysaccharide-stimulated tissue factor in human monocytes and monocyte-derived microvesicles

Immunomodulatory effects in humans of Δ⁹⁻Tetrahydrocannabinol (THC), the psychoactive component of marijuana are controversial. Tissue factor (TF), the activator of the extrinsic coagulation cascade, is increased...

Authors: Julie C. Williams, Thomas W. Klein, Bruce A. Goldberger, John W. Sleasman, Nigel Mackman and Maureen M. Goodenow

Sera from patients with active systemic lupus erythematosus patients enhance the toll-like receptor 4 response in monocyte subsets

Systemic Lupus Erythematosus (SLE) is an auto-immune disease whose complex pathogenesis remains unraveled. Here we aim to explore the inflammatory ability of SLE patients’ sera upon peripheral blood (PB) monoc...

Authors: Tiago Carvalheiro, Diane Gomes, Ligia A. Pinto, Luis Inês, Ana Lopes, Ana Henriques, Susana Pedreiro, António Martinho, Hélder Trindade, Howard A. Young, José António Pereira da Silva and Artur Paiva

Inflammasomes are important mediators of prostatic inflammation associated with BPH

There is mounting evidence to support the role of inflammation in benign prostate hyperplasia (BPH), and a recent study reported expression of inflammasome derived cytokine IL-18 in prostate biopsy of BPH pati...

Authors: Mahendra Kashyap, Subrata Pore, Zhou Wang, Jeffrey Gingrich, Naoki Yoshimura and Pradeep Tyagi

The PPAR-γ antagonist GW9662 elicits differentiation of M2c-like cells and upregulation of the MerTK/Gas6 axis: a key role for PPAR-γ in human macrophage polarization

The nuclear receptors PPAR-γ and LXRs regulate macrophage lipid metabolism and macrophage mediated inflammation. We examined the influence of these molecules on macrophage alternative activation, with particul...

Authors: Gaetano Zizzo and Philip L Cohen

Mucus mediated protection against acute colitis in adiponectin deficient mice

Acute ulcerative colitis is an inflammation-driven condition of the bowel. It hampers the general homeostasis of gut, resulting in decreased mucus production and epithelial cell renewal. Adiponectin (APN), an ...

Authors: Kamaljeet Kaur, Arpit Saxena, Bianca Larsen, Samantha Truman, Nathan Biyani, Emma Fletcher, Manjeshwar Shrinath Baliga, Venkatesh Ponemone, Shweta Hegde, Anindya Chanda and Raja Fayad

Glucose 6-phosphate dehydrogenase knockdown enhances IL-8 expression in HepG2 cells via oxidative stress and NF-κB signaling pathway

This study was designed to investigate the effect of glucose 6-phosphate dehydrogenase (G6PD) deficiency on pro-inflammatory cytokine secretion using a palmitate-induced inflammation HepG2 in vitro model. The ...

Authors: Hung-Chi Yang, Mei-Ling Cheng, Yi-Syuan Hua, Yi-Hsuan Wu, Hsin-Ru Lin, Hui-Ya Liu, Hung-Yao Ho and Daniel Tsun-Yee Chiu

Pentoxifylline reduces the inflammatory process in diabetic rats: relationship with decreases of pro-inflammatory cytokines and inducible nitric oxide synthase

Studies suggest that inflammation is a key factor in the pathogenesis of diabetes mellitus. Pro-inflammatory cytokines, such as IL-6 and TNF-alpha, are produced by adipose tissue in large quantities, in obese ...

Authors: Francisca Adilfa de Oliveira Garcia, Jéssica Farias Rebouças, Teresa Queiroz Balbino, Teresinha Gonçalves da Silva, Carlson Hélder Reis de Carvalho-Júnior, Gilberto Santos Cerqueira, Gerly Anne de Castro Brito and Glauce Socorro de Barros Viana

TLR2 and AP-1/NF-kappaB are involved in the regulation of MMP-9 elicited by heat killed Listeria monocytogenes in human monocytic THP-1 cells

MMP-9 is crucial for a normal immune response, but excessive release of this enzyme leads to severe tissue damage. Listeria monocytogenes (LM) is an opportunistic food-borne pathogen causing listerosis, meningiti...

Authors: Puthiyaveetil Kochumon Shihab, Areej Al-Roub, Moneera Al-Ghanim, Anfal Al-Mass, Kazem Behbehani and Rasheed Ahmad

ATF4 is a novel regulator of MCP-1 in microvascular endothelial cells

Monocyte chemoattractant protein-1 (MCP-1) is a major chemokine that recruits monocyte/macrophage to the site of tissue injury and plays a critical role in microvascular complications of diabetes. However, the...

Authors: Huibin Huang, Guangjun Jing, Joshua J Wang, Nader Sheibani and Sarah X Zhang

Cytomics of the tumour microenvironment: therapeutic targeting (keynote lecture)

Authors: Paul Smith

Bone marrow transplant model to study articular cartilage repair

Authors: Ana Sergijenko, Anke J Roelofs, Andrea Augello and Cosimo De Bari

Flow cytometric techniques for isolating and analysing leucocytes

Authors: Adriano G Rossi and Fiona Rossi

The effect of aged erythrocytes and erythrocyte-derived microparticles on lymphomatous and healthy B cells

Authors: Caitlin Hughes, Mark A Vickers, Robert N Barker and Lindsay S Hall

Translating research flow cytometry assays for characterization of cellular therapeutics

Authors: John DM Campbell

Can selenium supplementation modify oxidative stress in-vitro? A role for selenium supplementation in the prevention of cardiovascular disease

Authors: Dean Leighton, Marie Goua, Eimear Dolan, Katherine Burgess and Giovanna Bermano

Cell therapies for joint repair

Authors: Cosimo De Bari

TGF-β2 mediated secretion of sCTLA-4 from regulatory T cells

Authors: R Khanolkar, S Rajpara, F Muller, I Depasquale, L Lawson, RN Barker, M Nicolson, AD Ormerod and FJ Ward

Drug effects in multiple rounds of cell division monitored by flow cytometry

Authors: Derek Davies

Quality in clinical cytometry

Authors: David WL Wilson

Development of a FACS-based Smac/DIABLO release assay to monitor individual drug responses and to pave the way towards patient-tailored therapies

Authors: Jigyasa Arora, Ralf Michael Zwacka and Andrea Mohr

Characterisation of the murine C-type lectin receptor CLECSF8 (MCL) reveals its expression on cells of the monocyte/neutrophil lineages and an inter-dependence with Mincle, but not Dectin-2

Authors: Bernhard Kerscher, Gillian J Wilson, Delyth M Reid, Sho Yamasaki, Janet A Willment and Gordon D Brown

A unique approach to compensation and its verification

Authors: Ian Dimmick

FACS for Fungi: revealing population heterogeneity among fungal pathogens via flow cytometry

Authors: Elizabeth R Ballou and Alistair JP Brown

Characterisation of a novel vaccine delivery system for livestock

Authors: Rebecca K McLean, Ann R Wood, Jayne C Hope, Gary Entrican and David J Griffiths

CCL19-sorted mature dendritic cells have enhanced lymph node migratory capacity and function

Authors: Michelle L Le Brocq, Alasdair R Fraser, Gerard J Graham and John D M Campbell

Sharing the love: networking amongst cytometrists

Authors: Derek Davies

Deletion of myeloid-PTP1B decreases MHC Class I expression and peptide presentation through an IL-10 dependent mechanism in response to LPS challenge

Authors: Samantha Le Sommer, Cristina Martin-Granados and Mirela Delibegovic

Introduction and welcome

Authors: Raif Yuecel

Role of the NLRP3 inflammasome in the transient release of IL-1β induced by monosodium urate crystals in human fibroblast-like synoviocytes

To investigate whether monosodium urate (MSU) crystals induce interleukin (IL)-1β in human fibroblast-like synoviocytes (FLS), and whether the NLRP3 inflammasome is involved in the inflammatory mechanism.

Authors: Shu-cong Zheng, Xiao-xia Zhu, Yu Xue, Li-hong Zhang, He-jian Zou, Jian-hua Qiu and Qiong Liu

Adipokine gene expression in peripheral blood of adult and juvenile dermatomyositis patients and their relation to clinical parameters and disease activity measures

Recently adipokines have been implicated in the regulation of immune and inflammatory responses in autoimmune disease. To investigate the role of adipokines in adult and pediatric patients with newly diagnosed...

Authors: Jeannette M Olazagasti, Molly Hein, Cynthia S Crowson, Consuelo Lopez de Padilla, Erik Peterson, Emily C Baechler and Ann M Reed

Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren’s sarcoidosis patients

Sarcoidosis is a granulomatous disease affecting in particular the lungs. The peroxisome proliferator-activated receptors (PPARs) play important regulatory roles in inflammation. The aim of this study was to g...

Authors: Muntasir Abo Al Hayja, Anders Eklund, Johan Grunewald and Jan Wahlström

Valproic acid attenuates intercellular adhesion molecule-1 and E-selectin through a chemokine ligand 5 dependent mechanism and subarachnoid hemorrhage induced vasospasm in a rat model

Up-regulation of regulated upon activation, normal T-cell expressed and secreted (RANTES/CCL5) and adhesion molecules is observed in the serum of animals following experimental subarachnoid hemorrhage (SAH). T...

Authors: Chih-Zen Chang, Shu-Chuan Wu, Chih-Lung Lin and Aij-Lie Kwan

Chronic Kidney Disease is associated with an increase of Intimal Dendritic cells in a comparative autopsy study

Chronic Kidney Disease (CKD) and inflammation are risk factors for atherosclerotic vascular disease (ASVD). In inflammatory conditions, Nuclear Factor-κB (NF-κB) is frequently activated and it has been detecte...

Authors: Miguel Hueso, Joan Torras, Marta Carrera, August Vidal, Estanis Navarro and Josep Grinyó

Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients

We aimed to investigate peripheral blood eosinophil chemotaxis, generation of spontaneous reactive oxygen species (ROS), and apoptosis in patients with allergic asthma after bronchial allergen challenge.

Authors: Simona Lavinskiene, Kestutis Malakauskas, Jolanta Jeroch, Deimante Hoppenot and Raimundas Sakalauskas

Expression of hedgehog signal pathway in articular cartilage is associated with the severity of cartilage damage in rats with adjuvant-induced arthritis

Cartilage damage is a crucial step in rheumatoid arthritis (RA) disease progress while its molecular mechanisms are not fully understood. Here we investigated the expression of hedgehog (Hh) signal pathway in ...

Authors: Rong Li, Li Cai, Cheng-mu Hu, Ting-ni Wu and Jun Li

The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats

To explore the immunological mechanisms underlying the effect of chronic intermittent hypobaric hypoxia (CIHH) pretreatment on collagen-induced arthritis (CIA) in rat.

Authors: Min Shi, Fang Cui, Ai-Jing Liu, Hui-Jie Ma, Ming Cheng, Shu-Xia Song, Fang Yuan, De-Pei Li and Yi Zhang

TNF-alpha and IL-6 inhibit apolipoprotein A-IV production induced by linoleic acid in human intestinal Caco2 cells

Apolipoprotein A-IV (apoA-IV) is a protein mainly synthesized by enterocytes in the intestine. Its gene expression is suppressed during fasting and stimulated during active fat absorption. Chronic feeding of a...

Authors: Xiaoming Li, Min Xu, Min Liu, Yong Ji and Zongfang Li

Association of serum TNF-α, IL-8 and free light chain with HLA-DR B alleles expression in pulmonary and extra-pulmonary sarcoidosis

Sarcoidosis is a systemic disease of unknown etiology characterized histologically by the observation of non-caseating granulomas and several immunological abnormalities. Sarcoidosis is a multi-organ disorder ...

Authors: Esmaeil Mortaz, Hale Abdoli Sereshki, Atefeh Abedini, Arda Kiani, Mehdi Mirsaeidi, Dina Soroush, Johan Garssen, Aliakbar Velayati, Frank A Redegeld and Ian M Adcock

Neutrophil-to-lymphocyte ratio, calprotectin and YKL-40 in patients with chronic obstructive pulmonary disease: correlations and 5-year mortality – a cohort study

Chronic obstructive pulmonary disease (COPD) is characterized by chronic inflammation and progressive decline in pulmonary function. Neutrophil-to-lymphocyte ratio (NLR), YKL-40 and calprotectin are biomarkers...

Authors: Allan Klitgaard Sørensen, Dennis Back Holmgaard, Lone Hagens Mygind, Julia Johansen and Court Pedersen

Crude extract of hydatid laminated layer from Echinococcus granulosus cyst attenuates mucosal intestinal damage and inflammatory responses in Dextran Sulfate Sodium induced colitis in mice

Inflammatory bowel disease is an immunologically mediated disease. Notably, it is less common in countries where there is a greater risk of exposure to helminths. In our study, we examined the modulatory effec...

Authors: Imene Soufli, Ryma Toumi, Hayet Rafa, Manel Amri, Moussa Labsi, Lila Khelifi, Ferdinando Nicoletti and Chafia Touil-Boukoffa

MicroRNA-181b regulates endotoxin tolerance by targeting IL-6 in macrophage RAW264.7 cells

Interleukin 6 (IL-6) is a major pro-inflammatory cytokine and dysregulation of IL-6 is relevant to many inflammatory diseases. Endotoxin induced tolerance of IL-6 is an important mechanism to avoid the excessi...

Authors: Wenjun Zhang, Xiaojun Shen, Luyang Xie, Maoping Chu and Yanmei Ma

Anti-inflammatory effects of an injectable copolymer of fatty acids (Ara 3000 beta®) in joint diseases

In inflammatory joint disease, such as osteoarthritis or arthritis, there is an increased level of pro-inflammatory cytokines, such as interleukin-1β. These cytokines stimulate the expression and release of ma...

Authors: Catherine Baugé, Eva Lhuissier, Nicolas Girard, Céline Quesnelle, Gérard Ewert and Karim Boumediene

Human parainfluenza virus type 3 (HPIV3) induces production of IFNγ and RANTES in human nasal epithelial cells (HNECs)

Human parainfluenza virus type 3 (HPIV3), while infecting lower airway epithelial cells induces pneumonia and bronchiolitis in infants and children, and may lead to asthma exacerbations in children and adults....

Authors: Anna Lewandowska-Polak, Małgorzata Brauncajs, Edyta Paradowska, Marzanna Jarzębska, Marcin Kurowski, Sylwia Moskwa, Zbigniew J Leśnikowski and Marek L Kowalski